Coding
Part:BBa_K3590020:Design
Designed by: Barbora Hrnčířová Group: iGEM20_Brno_Czech_Republic (2020-10-18)
3x LysM domain derived from Bacillus subtilis LytE version 1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 325
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 325
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 325
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 325
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 387
Design Notes
The sequence was codon-optimized for easier gene synthesis.
Source
Accession: U38819, nucleotides 515 to 1132. The sequence was codon-optimized for easier gene synthesis and a few codons have been changed in version 2 to avoid homologous recombination between the linkers in one construct.